Pretreatment of Paddy Straw for Enhanced Saccharification

This is BioTechnology project report on "pretreatment of paddy straw for enhanced saccharification". This project deals with enhanced saccharification of paddy straw by optimizing numerous physical and chemical pre-treatments like a steam explosion, temperature, and treatment with hydrogen chloride, a highly electronegative acid. The amount of breakdown of lignin, hemicellulose & cellulose was determined using Fourier Transform Infrared Spectroscopy (FTIR). You can also subscribe to FINAL YEAR PROJECT'S by Email for more such projects and seminar.

The maximum quantity of breakdown of a polymer is discovered for the 90 minutes long at frequency 1462.88cm-1 and most exposure of xylan is discovered at frequency 1602.9cm-1for ninety minutes exposure to steam explosion. In coupled pretreatment methodology (steam explosion & acidic at the same time given) most polyose breakdown came about once acidic pretreatment followed by a steam explosion that was discovered by FTIR at 1320.32 cm-1 frequency at the same time microscopic anatomy changes were determined by staining and mental image at 10X exploitation saffranin dye.

Biomass with high lignocellulosic content has the maximum potential for the production of biofuels such as Butanol, Ethanol, etc. Conversion of lignocelluloses to a biofuel is multi-step process which includes the following processes: Mechanical Disintegration, Physical pretreatment, Chemical Hydrolysis, Enzymatic saccharification & Fermentation.

Fourier transform infrared (FTIR) spectroscopy is a rapid and quantitative technique for the identification of components of solid or lingo-cellulose in the mid-IR region. The fact that pretreatment is given before fermenting sample it is to carry out saccharification of disaccharides and carbohydrates present in lignocelluloses biomass. Pretreatment methods refer to the solubilization and separation of one or more of these components of biomass. It makes the remaining solid biomass more accessible to further chemical or biological treatment.

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Lignocellulosic degradation for the formation of monomers of glucose has always been an initial step for biofuel production processes. Whereas effective degradation of lignocellulosic biomass can only be achieved by cheap and effective pretreatment or saccharification processes.

It can be concluded from the following study that to obtain the maximum degradation of lignin in the future raw material is recommended to be steam exploded for 90 minutes at 121°C|15psi then acidic exploded with 1N hydrochloric acid for 60 minutes at 121°C|15 psi followed by co-culture enzymatic hydrolysis with electrostimulated. Use this project report for your reference and study only.


Author:- Vipul Swarup Bhatnagar

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